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Biology

Biotechnology: Principles and Processes

Restriction enzymes, cloning vectors like pBR322, gel electrophoresis, PCR, and bioreactors form the toolkit, tied together by the steps of recombinant DNA technology. Expect recall plus process questions: vector site maps, step sequences, and tool functions.

Exam rules change between cycles. Confirm dates, syllabus and pattern against NTA's official site. We are not NTA.

Weightage: what the paper actually did

Biotechnology: Principles and Processes carried 6 questions in the NEET 2026 paper. One paper is a data point, not a destiny: NTA moves weight between chapters every cycle, which is why the weightage tables deserve care. The stable facts are the paper's shape: 180 questions, 720 marks, +4 right, −1 wrong.

Sample PYQs from this chapter

Real questions NTA asked from Biotechnology: Principles and Processes, with the answer and the working. Try before you peek.

Question 1 · NEET 2026

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
  1. A.Extension →\rightarrow Annealing →\rightarrow Denaturation
  2. B.Annealing →\rightarrow Denaturation →\rightarrow Extension
  3. C.Denaturation →\rightarrow Extension →\rightarrow Annealing
  4. D.Denaturation →\rightarrow Annealing →\rightarrow Extension
Answer and explanation

Answer: D

Each PCR cycle proceeds as denaturation (heat separates the two DNA strands), then annealing (primers bind to the single-stranded templates), then extension (DNA polymerase synthesises the new strands). Hence the correct order is Denaturation, then Annealing, then Extension. Why the other options are wrong: - (A) Extension cannot precede strand separation and primer binding. - (B) Annealing needs single strands, so denaturation must come first. - (C) Extension follows annealing, not the reverse.

Question 2 · NEET 2026

Arrange the following steps of somatic hybridisation in the correct sequence and choose the correct answer from the options given below: I. A. Digestion of cell walls. II. B. Isolation of naked protoplasts. III. C. Fusion of protoplasts to get hybrid protoplast. IV. D. Isolation of single cells from two different varieties of plants. V. E. Growing of hybrid protoplast to form a new plant.
  1. A.D, A, B, C, E
  2. B.E, B, A, D, C
  3. C.D, B, A, E, C
  4. D.E, A, B, C, D
Answer and explanation

Answer: A

Somatic hybridisation starts by isolating single cells from two different plant varieties (D), digesting their cell walls with enzymes (A) to release naked protoplasts (B), fusing the protoplasts to form a hybrid protoplast (C), and finally growing it to regenerate a new plant (E). Hence the correct sequence is D, A, B, C, E.

How to practise Biotechnology: Principles and Processes

Chapter by chapter, against real past-paper questions, on a screen: NEET is computer based from 2027. Read the chapter once, then attempt its PYQs until your accuracy stops climbing, and let the wrong answers tell you which lines to reread. If the chapter keeps refusing to stick, that has its own guide. There is also a free 12-question mock on the exam screen.

Nearby Biology chapters

Questions

How many questions did Biotechnology: Principles and Processes have in NEET 2026?

Biotechnology: Principles and Processes carried 6 questions in the NEET 2026 paper. Weightage shifts between cycles, so treat it as a probability, not a promise.

Where can I practise Biotechnology: Principles and Processes PYQs for NEET?

In the BrainIt app, free until the 2027 exam: its bank holds 50,000 past NTA questions tagged to all 83 chapters, including this one, with accuracy tracked per chapter. Sample questions from this chapter are on this page.

Is Biotechnology: Principles and Processes important for NEET 2027?

It is part of the Biology syllabus the paper draws from, and NEET 2027 is computer based, so practise it on a screen. Your own accuracy in the chapter matters more than any weightage table: a heavy chapter you already score well on needs less time than a light one you keep missing.

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